FLIP: a novel regulator of macrophage differentiation and granulocyte homeostasis.

FLIP: a novel regulator of macrophage differentiation and granulocyte homeostasis Running head: FLIP in myeloid cells

Qi-Quan Huang, Harris Perlman, Zan Huang, Robert Birkett, Lixin Kan, Hemant Agrawal, Alexander Misharin, Sandeep Gurbuxani , John D. Crispino, Richard M. Pope Divisions of Rheumatology and Hematology/Oncology, Department of Medicine, Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60691, USA; 4 Department of Pathology, University of Chicago. Chicago, I...

PHAGOCYTES, GRANULOCYTES, AND MYELOPOIESIS FLIP: a novel regulator of macrophage differentiation and granulocyte homeostasis

FLIP is a well-established suppressor of death receptor-mediated apoptosis. To define its essential in vivo role in myeloid cells, we generated and characterized mice with Flip conditionally deleted in the myeloid lineage. Myeloid specific Flip-deficient mice exhibited growth retardation, premature death, and splenomegaly with altered architecture and extramedullary hematopoiesis. They also dis...

Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)

Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...

Hepcidin Induces M1 Macrophage Polarization in Monocytes or THP-1 Derived Macrophages

Background: Macrophage polarization plays a critical role in determining the inflammatory states. Hepcidin is a key negative regulator of iron homeostasis and functions. Although hepcidin has been shown to affect ferroportin expression in macrophages, whether it affects macrophage polarization is still largely unknown. Objective: To address whether hepcidin ind...

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli